RSS feed source: National Science Foundation
NSF 24-120
August 28, 2024
Dear Colleagues:
Plant genetic transformation, a process of introducing DNA, RNA, and proteins into plant cell/tissue and the regeneration of transformed materials, is of vital importance for both basic and applied research. In basic research, the generation of knock-out mutants, targeted mutagenesis, or over-expressing lines by plant transformation is a key approach for the functional characterization of genes. In applied research, genetic transformation enables genome editing and transgenesis that allows precise and knowledge-based gene modifications for plant breeding.
Except for Arabidopsis thaliana and a few other species from the Brassicaceae family, which can be transformed using non-tissue culture-based technique, most plant species require complex transformation and regeneration protocols with extensive in vitro culture procedures. These protocols are time-consuming, expensive, and often technically demanding. For many plant species, regeneration
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